Leslie C Plhak

US Patent:

6444433, Sep 3, 2002

Filed:

Jan 24, 2000

Appl. No.:

09/490547

Inventors:

Leslie C. Plhak - Middleton WI
Eun-Sung Park - Galveston TX

Assignee:

Board of Supervisors of Louisiana State University and Agriculture and Mechanical College - Baton Rouge LA

International Classification:

G01N 33543

US Classification:

435 792, 435 71, 436547, 436548, 5303881

Abstract:

We have produced a hybridoma cell line that produces monoclonal antibodies (MAb) that bind effectively to 2-methylisoborneol (MIB). The MAbs were produced using a structurally related molecule, borneol, whose structure was minimally changed when complexed to the protein carrier. These MAbs detected MIB at approximately 0. 01 to 1 ppb (or lower) when used in ELISA assays. Because these antibodies were produced as MAbs, their affinity characteristics remained constant. With access to MAbs, MIB can be easily detected at low levels using immunological techniques that are adapted to fast and easy field assays of large samples. For example, catfish could easily be assayed in the field for the presence of MIB prior to marketing.