Vinod B Makhijani

US Patent:

7107987, Sep 19, 2006

Filed:

Feb 10, 2004

Appl. No.:

10/775465

Inventors:

Shivshankar Sundaram - Madison AL, US
Balabhaskar Prabhakarpandian - Madison AL, US
Vinod Makhijani - Guilford CT, US
Andrzej Przekwas - Huntsville AL, US

Assignee:

CFD Research Corporation - Huntsville AL

International Classification:

A61M 11/00
A61M 15/00

US Classification:

12820023, 12820022, 12820315

Abstract:

A spacer for delivering a medication spray from an inhaler includes a first conical body joined to a second conical body, forming a continuous spray conduit through first and second internal chambers of the respective first and second conical bodies. A mouthpiece is formed in the proximal end of the first conical body. A spray inlet for attachment to the inhaler is formed at the distal end of the second conical body. A plurality of air inlets are placed downstream of the medication inlet proximate to, or in, the large diameter distal end surface of the first conical body. Recirculation zones are created in the first and second chambers, to force the medication spray into a central airflow path through the spray conduit, minimizing particle deposition by contract with the walls of the spacer.

US Patent:

7189578, Mar 13, 2007

Filed:

Dec 2, 2002

Appl. No.:

10/307907

Inventors:

Jianjun Feng - Huntsville AL, US
Sivaramakrishnan Krishnamoorthy - Huntsville AL, US
Vinod Bhagwan Makhijani - Huntsville AL, US

International Classification:

G01N 1/00
G01N 1/10
G01N 35/00
H05F 3/00
B01D 57/02

US Classification:

436174, 422 50, 422 681, 422 81, 422 82, 422100, 422101, 422102, 422104, 29592, 295921, 436 43, 436180, 204164, 204450, 204458, 204477

Abstract:

A method and system for controlling flow motion in a channel/cavity in a microfluidic system includes positioning at least one pair of electrodes in and/or proximate to the channel/cavity. A buffer solution is placed in the channel/cavity, the buffer solution having at least one dielectric property that varies in response to changes in temperature of the solution. An AC/DC voltage is applied to the electrodes to generate an electric field in the channel/cavity; the AC voltage having a known magnitude and frequency and the DC voltage having a known magnitude. The magnitude of the AC/DC voltage is adjusted to cause Joule heating of the buffer solution in the channel/cavity. The geometry and position of the electrodes is adjusted to generate a temperature gradient in the buffer solution, thereby causing a non-uniform distribution of the dielectric property within the solution in the channel/cavity. The dielectric non-uniformity produces a body force and flow in the solution. Also, the frequency of the AC voltage is adjusted to generate flow of the buffer solution in the channel/cavity in response to the non-uniform distribution of the dielectric property.

US Patent:

7842457, Nov 30, 2010

Filed:

Jan 28, 2004

Appl. No.:

10/767899

Inventors:

Jan Berka - New Haven CT, US
Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Steve Lefkowitz - Branford CT, US
Kenton L. Lohman - Guilford CT, US
Vinod B. Makhijani - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Gary J. Sarkis - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 912

Abstract:

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

US Patent:

8012690, Sep 6, 2011

Filed:

Oct 31, 2007

Appl. No.:

11/982095

Inventors:

Jan Berka - New Haven CT, US
Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Steve Lefkowitz - Branford CT, US
Kenton L. Lohman - Guilford CT, US
Vinod B. Makhijani - Guilford CT, US
Jonathan M. Rothberg - Guilford CT, US
Gary J. Sarkis - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

Assignee:

454 Life Sciences Corporation - Branford CT

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 612, 435 912

Abstract:

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

US Patent:

20060019264, Jan 26, 2006

Filed:

Nov 23, 2004

Appl. No.:

11/016942

Inventors:

Said Attiya - New Haven CT, US
Vinod Makhijani - Guilford CT, US
Ming Lei - Madison CT, US
Yi-Ju Chen - New Haven CT, US
John Simpson - Madison CT, US
C. Roth - Fairfield CT, US
Chun Ho - East Haven CT, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435006000, 435287200

Abstract:

The present invention relates to methods and apparati for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification.

US Patent:

20060088857, Apr 27, 2006

Filed:

Sep 1, 2005

Appl. No.:

11/217194

Inventors:

Said Attiya - New Haven CT, US
Vinod Makhijani - Guilford CT, US
Ming Lei - Madison CT, US
Yi-Ju Chen - New Haven CT, US
John Simpson - Madison CT, US
G. Roth - Fairfield CT, US
Chun Ho - East Haven CT, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435006000, 435287200

Abstract:

The present invention relates to methods and apparatuses for conducting densely packed, independent chemical reactions in parallel in fluid-permeable arrays. Accordingly, this invention also focuses on the use of such arrays for applications such as DNA sequencing, most preferably pyrophosphate sequencing, and DNA amplification.

US Patent:

20110201526, Aug 18, 2011

Filed:

Feb 23, 2011

Appl. No.:

13/033240

Inventors:

Jan Berka - New Haven CT, US
Yi-Ju Chen - New Haven CT, US
John H. Leamon - Guilford CT, US
Steve Lefkowitz - Branford CT, US
Kenton L. Lohman - Guilford CT, US
Vinod B. Makhijani - Guilford CT, US
Johathan M. Rothberg - Guilford CT, US
Gary J. Sarkis - Guilford CT, US
Maithreyan Srinivasan - Branford CT, US
Michael P. Weiner - Guilford CT, US

International Classification:

C40B 50/06

US Classification:

506 26

Abstract:

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

US Patent:

20110287432, Nov 24, 2011

Filed:

May 18, 2011

Appl. No.:

13/110589

Inventors:

Chiu Tai Andrew Wong - Northford CT, US
Yi-Ju Chen - New Haven CT, US
Vinod Bhagwan Makhijani - Guilford CT, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435 612, 4352872

Abstract:

An embodiment of a method for optimizing sequencing performance is described that comprises the steps of calculating a nucleotide species specific degradation rate of an apyrase enzyme for a plurality of nucleotide species; determining a concentration for each of the nucleotide species using the nucleotide species specific degradation rate; iteratively providing the concentration of each of the nucleotide species in a reaction environment comprising a polymerase enzyme and a species of template nucleic acid molecule, wherein one or more molecules of the nucleotide species are incorporated into a nascent molecule in a sequencing reaction and the apyrase enzyme is introduced to the reaction environment to degrade unincorporated nucleotide species molecules; and detecting a signal in response to the incorporation of the nucleotide species.