Dow AgroSciences LLC - Indianapolis IN, US Diaa Alabed - Carmel IN, US Sara Bennett - Indianapolis IN, US Manju Gupta - Carmel IN, US Susan Jayne - Zionsville IN, US Terry R. Wright - Carmel IN, US
Assignee:
DOW AGROSCIENCES LLC - Indianapolis IN
International Classification:
C12N 15/82
US Classification:
800278, 536 241, 435468, 435419, 4353201
Abstract:
Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
Susan Jayne - Des Moines IA Eric Barbour - Des Moines IA Terry Meyer - Urbandale IA
Assignee:
Pioneer Hi-Bred International, Inc. - Johnston IA
International Classification:
C12N 504 C12N 1511 C12N 1582 A01H 500 A01H 510
US Classification:
800300
Abstract:
The present invention is drawn to compositions and methods for improving transformation efficiency. The compositions, synthetic marker genes, are used in transformation methods and result in increased transformation efficiency. The synthetic marker genes can be designed for maximum expression in any system.
- Indianapolis IN, US Manju GUPTA - Carmel IN, US Terry R. WRIGHT - Westfield IN, US Susan M. JAYNE - Zionsville IN, US Doug A. SMITH - Noblesville IN, US Diaa ALABED - Carmel IN, US
International Classification:
C12N 15/82 C12N 15/10
Abstract:
The c.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different species, v2.
Genetic Loci Associated With Culture And Transformation In Maize
- Indianapolis IN, US Susan M. Jayne - Indianapolis IN, US Diaa Alabed - Carmel IN, US Stephen Foulk - Carmel IN, US Rajat Aggarwal - Zionsville IN, US Natae Daniels - Brownsburg IN, US
This invention relates to methods for identifying maize plants that having increased culturability and/or transformability. The methods use molecular markers to identify and to select plants with increased culturability and/or transformability or to identify and deselect plants with decreased culturability and/or transformability. Maize plants generated by the methods of the invention are also a feature of the invention.
- Indianapolis IN, US Manju GUPTA - Carmel IN, US Terry R. WRIGHT - Westfield IN, US Susan M. JAYNE - Zionsville IN, US Doug A. SMITH - Noblesville IN, US Diaa ALABED - Carmel IN, US
International Classification:
C12N 15/82 C12N 15/10
Abstract:
The c.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different species, v2.
- INDIANAPOLIS IN, US Manju GUPTA - Carmel IN, US Terry R. WRIGHT - Carmel IN, US Susan M. JAYNE - Zionsville IN, US Doug A. SMITH - Noblesville IN, US Diaa ALABED - Carmel IN, US
International Classification:
C12N 15/82 C12N 15/10
Abstract:
The c.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different genotype, c.v. B104.
- INDIANAPOLIS IN, US Manju GUPTA - Carmel IN, US Terry R. WRIGHT - Carmel IN, US Susan M. JAYNE - Zionsville IN, US Doug A. SMITH - Noblesville IN, US Diaa ALABED - Carmel IN, US
International Classification:
C12N 15/82 C12N 15/10
Abstract:
The c.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different species, v2.
- INDIANAPOLIS IN, US Manju GUPTA - Carmel IN, US Terry R. WRIGHT - Carmel IN, US Susan M. JAYNE - Zionsville IN, US Doug A. SMITH - Noblesville IN, US Diaa ALABED - Carmel IN, US
International Classification:
C12N 15/82
Abstract:
The c.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different genotype, c.v. LLN37.
Intellectual Property Portfolio Manager at Dow AgroSciences
Location:
Indianapolis, Indiana Area
Industry:
Biotechnology
Work:
Dow AgroSciences since Apr 2012
Intellectual Property Portfolio Manager
Dow AgroSciences Jun 2007 - Mar 2012
Research Leader
Midwest Research Institute 2005 - 2007
Program Manager
Independent Consultant 2001 - 2007
Plant Transformation Subject Matter Expert
Pioneer Hi-Bred International 1993 - 2001
Research Coordinator
Education:
California State University-Fresno
B.S., Plant Science/International Agriculture
Skills:
Genetics Plant Breeding Crop Lifesciences Technology Transfer Molecular Biology Agriculture Plant Tissue Culture Plant Transformation Biotechnology DNA