Evgueni Ananiev - Johnston IA, US Mark A. Chamberlin - Windsor Heights IA, US William J. Gordon-Kamm - Urbandale IA, US Sergei Svitashev - Johnston IA, US Chengcang Wu - Johnston IA, US
Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
Evgueni Ananiev - Johnston IA, US Olga N. Danilevskaya - Johnston IA, US Mark A. Chamberlin - Windsor Heights IA, US William J. Gordon-Kamm - Urbandale IA, US Sergei Svitashev - Johnston IA, US Chengcang C. Wu - Middleton WI, US
Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
EVGUENI ANANIEV - JOHNSON IA, US Olga N. Danilevskaya - Johnston IA, US MARK A. CHAMBERLIN - WINDSOR HEIGHTS IA, US WILLIAM J. GORDON-KAMM - URBANDALE IA, US SERGEI SVITASHEV - Johnston IA, US CHENGCANG WU - Middleton IA, US
Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
Methods For Producing A Complex Transgenic Trait Locus
Michael Lassner - Urbandale IA, US Derek Jantz - Durham NC, US James Jefferson Smith - Durham NC, US Mark Cigan - Johnston IA, US Carl Falco - Wilmington DE, US Huirong Gao - Johnston IA, US Zhongsen Li - Hockessin DE, US Sergei Svitashev - Johnston IA, US
Assignee:
E.I. du Pont de Nemours and Company - Wilmington DE Pioneer Hi-Bred International, Inc. - Johnston IA
International Classification:
C12N 15/82
US Classification:
800270, 536 236, 800298, 4353201
Abstract:
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
Evgueni Ananiev - Johnston IA, US Mark A. Chamberlin - Windsor Heights IA, US William J. Gordon-Kamm - Urbandale IA, US Sergei Svitashev - Johnston IA, US Chengcang Wu - Johnston IA, US
Assignee:
Pioneer Hi-Bred International, Inc. - Johnston IA
International Classification:
A01H 5/00 C12N 15/82 C12N 5/04 C07H 21/04
US Classification:
800278, 8003201, 435412, 435468, 536 236
Abstract:
Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
Donor Design Strategy For Crispr-Cas9 Genome Editing
- JOHNSTON IA, US SATISH KANUGANTI - HYDERABAD, IN AMITABH MOHANTY - JOHNSTON IA, US SERGEI SVITASHEV - JOHNSTON IA, US VENKATA S. TAVVA - HYDERABAD, IN
Assignee:
PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA
International Classification:
C12N 15/10 C12N 15/82
Abstract:
Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, using concatemers of heterologous polynucleotides that are flanked by sequences capable of sequence hybridization with a guide RNA. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a heterologous polynucleotide as a repair template.
Use Of Morphogenic Factors For The Improvement Of Gene Editing
- JOHNSTON IA, US WILLIAM JAMES GORDON-KAMM - URBANDALE IA, US KEITH S LOWE - JOHNSTON IA, US DAVID J PETERSON - AMES IA, US SERGEI SVITASHEV - JOHNSTON IA, US JOSHUA K YOUNG - JOHNSTON IA, US
Assignee:
PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA
International Classification:
C12N 15/82
Abstract:
Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.
- JOHNSTON IA, US MAREN L ARLING - DES MOINES IA, US PIERLUIGI BARONE - DES MOINES IA, US WILLIAM JAMES GORDON-KAMM - URBANDALE IA, US SANDEEP KUMAR - JOHNSTON IA, US BRIAN LENDERTS - JOHNSTON IA, US KEITH S LOWE - JOHNSTON IA, US SERGEI SVITASHEV - JOHNSTON IA, US XINLI EMILY WU - JOHNSTON IA, US
Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.