Ramakrishna Seethala

US Patent:

7384757, Jun 10, 2008

Filed:

Mar 30, 2005

Appl. No.:

11/093428

Inventors:

Ramakrishna Seethala - West Windsor NJ, US
Dong Cheng - Furlong PA, US

Assignee:

Bristol-Myers Squibb Company - Princeton NJ

International Classification:

G01N 33/53
C12Q 1/48
C12N 9/00

US Classification:

435 76, 435 15, 435183

Abstract:

The present invention relates generally to enzyme assays and more particularly to a rapid, sensitive, reliable and robust homogeneous assay for acetyl CoA carboxylase activity comprising a coupled enzyme assay in a scintillation proximity format suitable for high-throughput screening. In one aspect, the assay couples ACC and FAS and the product, palmitic acid is then detected by scintillation counting. The invention also relates to the identification of modulators of ACC activity.

US Patent:

20080085528, Apr 10, 2008

Filed:

Oct 10, 2007

Appl. No.:

11/869778

Inventors:

Jovita Marcinkeviciene - Washington Crossing PA, US
Kevin O'Malley - Chalfont PA, US
Mark Kirby - New Hope PA, US
Aiying Wang - West Windsor NJ, US
Lisa Kopcho - Pemberton Borough NJ, US
Ramakrishna Seethala - West Windsor NJ, US

International Classification:

C12Q 1/48
C12Q 1/54

US Classification:

435014000, 435015000

Abstract:

The subject matter disclosed and claimed herein relates to novel in vitro assays for measuring glucokinase activity and use of these assays for identifying modulators of glucokinase.

US Patent:

20100311072, Dec 9, 2010

Filed:

Jan 28, 2009

Appl. No.:

12/864745

Inventors:

Ramakrishna Seethala - West Windsor NJ, US
Jessica Leihwa Dong - Brisbane CA, US
Kevin M. O'Malley - Chalfont PA, US

International Classification:

G01N 33/53
C12Q 1/68

US Classification:

435 6, 435 78, 435 76

Abstract:

The subject matter disclosed and claimed herein concerns measuring the binding affinity of glucokinase (“GK”) using a fluorescence polarization (“FP”) assay. The FP method includes use of modified GK ligands bound to a fluorescent label. Binding affinity is determined by measuring displacement of fluorescent ligand by the known or suspected GK ligands. The subject matter disclosed and claimed herein provides a robust high-throughput FP assay for the determination of binding affinity of ligands to glucokinase. The FP binding assay displayed both glucose and nucleotide dependence, and a useful dynamic range. The binding ICdata correlated well with GK activation ECdata.

Author:

Ramakrishna Seethala

ISBN #:

0824705629