Skills:
TECHNICAL SKILLS: I have 6 years of laboratory experience in the cGMP environment. Some of my technical skills include:<br/>Cellular Biology:<br/>Cell Culture, including maintaining Mammalian cell lines and design and perform cellular based assays including RNA extraction, quantifying RNA by nanodrop technology and real-time qPCR, Fluorescence Assays, Luminescence Assays, Cytotoxicity Assays such as MTT assay, trypan blue assay, XTT assay; Establishing and maintaining primary culture.<br/><br/>Biochemistry... estimation assays including BCA assay and Bradford Assay, Western Blots, ELISA, ELISpot, Microarrays, Immunohistocytochemistry, H/E staining, DNA isolation and transfection study, enzyme kinetic assay.<br/><br/>Ex Vivo:<br/>Establishing human placental explants from full term human placenta, tissue sampling, maintaining the ex-vivo system and protein extraction by tissue lysis.<br/><br/>In Vivo:<br/>Whole Animal Pharmacology, including drug administration (i.p), blood collection by cardiac puncture, organ sampling-liver, fetus, uterus, spleen.<br/><br/>Microscopy:<b... scanning electron and fluorescence microscopy.<br/><br/>Chromatog... layer chromatography (TLC), High performance liquid chromatography (HPLC)<br/><br/>Computers:<br/... PC and Mac Operation, Basic Web Designing (HTML), Microsoft Office Applications.<br/> Microsoft Office Suite, Graph Pad Prism, SPSS, Image J, Image Pro Premier, Sigma Plot, Proficient in Data Analysis.<br/> Adobe Photoshop CS6, Lightroom 4, Final cut Pro.