Phillip R Samayoa

US Patent:

20220175970, Jun 9, 2022

Filed:

Feb 21, 2019

Appl. No.:

16/971016

Inventors:

- Cambridge MA, US
Matthew G. Stanton - Cambridge MA, US
Matt Chiocco - Cambridge MA, US
Mark D. Angelino - Cambridge MA, US
Robert M. Kotin - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US

International Classification:

A61K 48/00
C12N 15/86

Abstract:

Provided herein are methods and constructs comprising close-ended DNA (ceDNA vectors) for maintaining or sustaining a level of transgene expression at a predetermined level or range for a predefined time, or increasing the level of transgene expression in a cell or a subject, where the transgene expression level can be modulated (e.g., increased) with one or more subsequent administrations (e.g., a re-dose or a booster administration) after an initial priming administration. Provided are methods for personalizing gene therapy throughout an individuals' lifespan to express a transgene at a level that meets an individual's needs, by modulating expression levels of a transgene expressed by ceDNA vector incrementally, or in a step-by-step manner, with one or more administrations after an initial priming administration (e.g., at time 0), thereby enabling titration of the level of expression of the transgene to a desired predetermined expression level or to a desired expression level range.

US Patent:

20220177545, Jun 9, 2022

Filed:

Mar 13, 2020

Appl. No.:

17/437123

Inventors:

- Cambridge MA, US
Debra Klatte - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Nathaniel Silver - Cambridge MA, US

International Classification:

C07K 14/755
C12N 15/85
A61K 48/00

Abstract:

The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding FVIII protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of FVIII protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of FVIII protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing FVIII protein. Such FVIII protein can be expressed for treating disease, e.g., hemophilia A.

US Patent:

20230024354, Jan 26, 2023

Filed:

Mar 13, 2020

Appl. No.:

17/437172

Inventors:

- Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Nathaniel Silver - Cambridge MA, US
Matthew Chiocco - Cambridge MA, US

International Classification:

A61K 48/00
C12N 15/85
C12N 9/02

Abstract:

The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding PAH protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of PAH protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of PAH protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing PAH protein. Such PAH protein can be expressed for treating disease, e.g., Phenylketonuria (PKU).

US Patent:

20210363480, Nov 25, 2021

Filed:

Dec 17, 2020

Appl. No.:

17/124955

Inventors:

- Boston MA, US
- VIENNA, AT
Teresa BERNINGER - Vienna, AT
Stephane COMPANT - Vienna, AT
Angela SESSITSCH - Vienna, AT
Geoffrey VON MALTZAHN - Boston CA, US
Richard Bailey FLAVELL - Thousand Oaks CA, US
Gerardo V. TOLEDO - Belmont MA, US
Slavica DJONOVIC - Malden MA, US
Luis Miguel MARQUEZ - Belmont MA, US
David Morris JOHNSTON - Cambridge MA, US
Yves Alain MILLET - Newtonville MA, US
Jeffrey LYFORD - Hollis NH, US
Jonathan W. LEFF - Cambridge MA, US
Phillip SAMAYOA - Cambridge MA, US
Craig SADOWSKI - Somerville MA, US

International Classification:

C12N 1/20
A01N 63/20
A01H 5/00
A01N 63/30
G01N 33/00

Abstract:

The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of interest, and to create libraries of microbes.

US Patent:

20210076685, Mar 18, 2021

Filed:

Mar 23, 2020

Appl. No.:

16/827551

Inventors:

- Boston MA, US
Nathan A. BILLINGS - Topsfield MA, US
Slavica DJONOVIC - Malden MA, US
Richard Bailey FLAVELL - Thousand Oaks CA, US
Trudi A. GULICK - Topsfield MA, US
David Morris JOHNSTON - Cambridge MA, US
Jonathan W. LEFF - Cambridge MA, US
Stephanie M. LIVA - Arlington MA, US
Jeffrey LYFORD - Hollis NH, US
Geoffrey VON MALTZAHN - Boston MA, US
Luis Miguel MARQUEZ - Belmont MA, US
Yves Alain MILLET - Newtonville MA, US
Craig SADOWSKI - Somerville MA, US
Phillip SAMAYOA - Cambridge MA, US
Gerardo V. TOLEDO - Belmont MA, US
David R. WEISMAN - Somerville MA, US
Xuecheng ZHANG - Newton MA, US

International Classification:

A01N 63/30
C05F 11/08
A01N 37/46
A01N 63/10

Abstract:

Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

US Patent:

20200306342, Oct 1, 2020

Filed:

Mar 16, 2020

Appl. No.:

16/819817

Inventors:

- Cambridge MA, US
Brett Boghigian - Boston MA, US
Caitlyn Harvey - Wilmington MA, US
David Berry - Brookline MA, US
David Young - Portsmouth NH, US
Geoffrey Von Maltzahn - Boston MA, US
John Kramarczyk - Somerville MA, US
Jameson Rogers - Cambridge MA, US
Kathryn Heard - Watertown MA, US
Michael Doherty - Somerville MA, US
Nathaniel Silver - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Subhayu Basu - Chestnut Hill MA, US
Shaila Rahman - Cambridge MA, US
Vimal Vaidya - Watertown MA, US

International Classification:

A61K 38/17
G16B 20/00
A61K 38/00
A23L 33/00
A23L 33/17
A23L 33/18
A23L 33/175
A61K 38/38
A61K 9/00
A61K 9/14
A61K 9/20
A61K 9/28
G01N 33/68
A23L 2/66
A61K 38/16
A61K 45/06
A23L 33/195
A61K 38/10
A61K 38/45

Abstract:

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

US Patent:

20200188481, Jun 18, 2020

Filed:

Sep 27, 2019

Appl. No.:

16/585357

Inventors:

- Cambridge MA, US
Brett Boghigian - Boston MA, US
Caitlyn Harvey - Wilmington MA, US
David Berry - Brookline MA, US
David Young - Portsmouth NH, US
Geoffrey Von Maltzahn - Boston MA, US
John Kramarczyk - Somerville MA, US
Jameson Rogers - Cambridge MA, US
Kathryn Heard - Watertown MA, US
Michael Doherty - Somerville MA, US
Nathaniel Silver - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Subhayu Basu - Chestnut Hill MA, US
Shaila Rahman - Cambridge MA, US
Vimal Vaidya - Watertown MA, US

International Classification:

A61K 38/17
G16B 20/00
A61K 38/00
A23L 33/00
A23L 33/17
A23L 33/18
A23L 33/175
A61K 38/38
A61K 9/00
A61K 9/14
A61K 9/20
A61K 9/28
G01N 33/68
A23L 2/66
A61K 38/16
A61K 45/06
A23L 33/195
A61K 38/10
A61K 38/45

Abstract:

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

US Patent:

20200008431, Jan 9, 2020

Filed:

Jul 15, 2019

Appl. No.:

16/512219

Inventors:

- Boston MA, US
- Vienna, AT
Teresa BERNINGER - Vienna, AT
Stephane COMPANT - Vienna, AT
Angela SESSITSCH - Vienna, AT
Geoffrey VON MALTZAHN - Boston CA, US
Richard Bailey FLAVELL - Thousand Oaks CA, US
Gerardo V. TOLEDO - Belmont MA, US
Slavica DJONOVIC - Malden MA, US
Luis Miguel MARQUEZ - Belmont MA, US
David Morris JOHNSTON - Cambridge MA, US
Yves Alain MILLET - Newtonville MA, US
Jeffrey LYFORD - Hollis NH, US
Jonathan W. LEFF - Cambridge MA, US
Phillip SAMAYOA - Cambridge MA, US
Craig SADOWSKI - Somerville MA, US

International Classification:

A01N 63/00
A01N 63/02
C12N 1/20
G01N 33/00
A01H 5/00

Abstract:

The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of interest, and to create libraries of microbes.