Peter U Konstantin

US Patent:

6849185, Feb 1, 2005

Filed:

May 12, 2000

Appl. No.:

09/959882

Inventors:

Xiaosong Wu - Pensacola FL, US
Michael Ishee - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Joel Shertok - Newark DE, US
Yujing Yang - Newton MA, US

International Classification:

B01D 6912

US Classification:

210660, 210488, 210490, 21050024, 21050027, 21050041, 2105021, 210504, 264 41, 427244, 427245, 427487, 436178, 521 27

Abstract:

The present invention provides a hydrophilic charged microporous membrane comprising a porous hydrophobic substrate and a coating comprising a charge-providing agent. The present invention further provides a hydrophilic charged membrane comprising a porous hydrophobic substrate and a hydrophilic charge-providing agent distributed within the substrate. The present invention further provides a filter as well as a filter device comprising the inventive hydrophilic charged membrane. The present invention further provides a process for treating a fluid containing bacterial contaminants such as endotoxins comprising contacting the membrane with the fluid to provide a bacterial contaminant depleted fluid.

US Patent:

6851561, Feb 8, 2005

Filed:

Jul 15, 2004

Appl. No.:

10/891098

Inventors:

Xiaosong Wu - Pensacola FL, US
Chung-Jen Hou - Pensacola FL, US
Jayesh Dharia - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D063/14
B01D063/06
B01D069/10
B01D069/12
B01D071/56

US Classification:

210490, 210435, 210488, 2104931, 21049701, 21050021, 21050037, 21050038

Abstract:

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

US Patent:

6860393, Mar 1, 2005

Filed:

Jul 21, 2004

Appl. No.:

10/895048

Inventors:

Chung-Jen Hou - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D063/14
B01D063/10
B01D067/00
B01D071/00

US Classification:

210435, 210483, 210490, 2104931, 21049701, 2104971, 21050035, 21050038, 21050041, 2105121, 264 41, 427244

Abstract:

The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e. g. , from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.

US Patent:

7094347, Aug 22, 2006

Filed:

Jan 18, 2005

Appl. No.:

11/035950

Inventors:

Xiaosong Wu - Pensacola FL, US
Chung-Jen Hou - Pensacola FL, US
Jayesh Dharia - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D 63/00
B01D 63/08
B01D 39/16
B01D 29/46
B01D 63/14

US Classification:

21032184, 21032188, 21050035, 21050037, 21050038, 210488, 210490, 2104931, 21032186

Abstract:

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

US Patent:

7132049, Nov 7, 2006

Filed:

Jan 19, 2005

Appl. No.:

11/037356

Inventors:

Chung-Jen Hou - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D 39/00
B01D 63/00
B01D 11/00
C02F 1/44

US Classification:

21050035, 21032175, 21032177, 2103218, 2104931, 21050038

Abstract:

The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e. g. , from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.

US Patent:

7189322, Mar 13, 2007

Filed:

Jan 26, 2005

Appl. No.:

11/042512

Inventors:

Xiaosong Wu - Pensacola FL, US
Michael Ishee - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Joel Shertok - Pace FL, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D 63/00
C02F 1/44
B01D 61/00

US Classification:

21032177, 21032175, 2103218, 21032186, 21032187, 2103216, 2104831

Abstract:

The present invention provides a hydrophilic charged microporous membrane comprising a porous hydrophobic substrate and a coating comprising a charge-providing agent. The present invention further provides a hydrophilic charged membrane comprising a porous hydrophobic substrate and a hydrophilic charge-providing agent distributed within the substrate. The present invention further provides a filter as well as a filter device comprising the inventive hydrophilic charged membrane. The present invention further provides a process for treating a fluid containing bacterial contaminants such as endotoxins comprising contacting the membrane with the fluid to provide a bacterial contaminant depleted fluid.

US Patent:

7223341, May 29, 2007

Filed:

Jul 6, 2006

Appl. No.:

11/480977

Inventors:

Xiaosong Wu - Pensacola FL, US
Chung-Jen Hou - Pensacola FL, US
Jayesh Dharia - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D 39/14
B01D 63/00

US Classification:

21050037, 21050021, 21050035, 21050038, 21050041, 210490

Abstract:

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

US Patent:

7396465, Jul 8, 2008

Filed:

May 1, 2007

Appl. No.:

11/742749

Inventors:

Xiaosong Wu - Pensacola FL, US
Chung-Jen Hou - Pensacola FL, US
Jayesh Dharia - Pensacola FL, US
Peter Konstantin - Boulder CO, US
Yujing Yang - Newton MA, US

Assignee:

Pall Corporation - East Hills NY

International Classification:

B01D 71/26
B01D 71/28
B01D 71/40
B01D 71/44
B01D 71/06

US Classification:

21050021, 21050037, 21050038, 21050041

Abstract:

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.