Lei Wu

US Patent:

6403367, Jun 11, 2002

Filed:

Dec 22, 1999

Appl. No.:

09/470448

Inventors:

Jing Cheng - Bejing, CN
Lei Wu - San Diego CA
Michael J. Heller - Encinitas CA
Ed Sheldon - Arcadia CA
Jonathan Diver - San Diego CA
James P. OConnell - Solana Beach CA
Dan Smolko - Jamul CA
Shila Jalali - San Diego CA
David Willoughby - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12M 134

US Classification:

4352871, 4352872, 4352885, 204545, 204547, 204403, 204409, 204643, 422 81, 422 8201

Abstract:

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a âlaboratory-on-a-chipâ system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

US Patent:

6448794, Sep 10, 2002

Filed:

Aug 22, 2000

Appl. No.:

09/643362

Inventors:

Jing Cheng - Beijing, CN
Junquan Xu - Fujian, CN
Xiaoshan Zhu - Hubei, CN
Litian Liu - Beijing, CN
Xiao-Bo Wang - San Diego CA
Lei Wu - San Diego CA

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

G01R 2708

US Classification:

324693, 324692, 324521, 324515, 349 33, 436806

Abstract:

The present invention concerns a high throughput electrorotation chip having an array of electrorotation units and methods of use thereof. To make the high throughput electrorotation chip, a plurality of electrorotation units (EU) are fabricated on a substrate or support and each EU is capable of producing a rotating electric field upon the application of an appropriate electrical signal. Exemplary embodiments include a row-column configuration of EUs having four electrode elements realized through two conductive-layers. The electrode elements may be linear, concave, or convex. Thin plates having one or multiple holes are bound to high-throughput electrorotation chips to form assay chambers having one or multiple wells. Particles can be introduced to the wells and electrorotation measurements can be performed on the particles. The high throughput electrorotation chip and chamber may be used for cell-based screening for leading drug candidate molecules from a compound library, for high-throughput characterizing particle electric properties, and for high-throughput assaying molecular compositions of unknown solutions.

US Patent:

6596143, Jul 22, 2003

Filed:

Oct 3, 2000

Appl. No.:

09/678263

Inventors:

Xiao-Bo Wang - San Diego CA
Weiping Yang - San Diego CA
Junquan Xu - Fujian, CN
Jing Cheng - Beijing, CN
Lei Wu - San Diego CA

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

G01N 2726

US Classification:

204547, 204643, 204450

Abstract:

A device acts as a particle switch to transport and/or re-direct microparticles which are in a fluid suspension. The switch comprises at least three structural branches and the branches may be connected at a common junction. Particles can be transported along the branches as a result of the forces generated along that branch. Particles are transported into or out of the particle switch via the ends of the branches. Particles can be switched from one branch into one of the other branches. Depending on the properties of the particles, the transportation mechanism may be traveling-wave-dielectrophoresis or traveling-wave-electrophoresis.

US Patent:

6630835, Oct 7, 2003

Filed:

Aug 7, 2002

Appl. No.:

10/216003

Inventors:

Jing Cheng - Beijing, CN
Junquan Xu - Fujian, CN
Xiaoshan Zhu - DanYang, CN
Litian Liu - Beijing, CN
Xiao-Bo Wang - San Diego CA
Lei Wu - San Diego CA

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

G01R 2708

US Classification:

324693, 324692, 324696, 324770, 20440301, 4351731, 435 4, 436809, 349 32

Abstract:

The present invention concerns a high throughput electrorotation chip having an array of electrorotation units and methods of use thereof. To make the high throughput electrorotation chip, a plurality of electrorotation units (EU) are fabricated on a substrate or support and each EU is capable of producing a rotating electric field upon the application of an appropriate electrical signal. Exemplary embodiments include a row-column configuration of EUs having four electrode elements realized through two conductive-layers. The electrode elements may be linear, concave, or convex. Thin plates having one or multiple holes are bound to high-throughput electrorotation chips to form assay chambers having one or multiple wells. Particles can be introduced to the wells and electrorotation measurements can be performed on the particles. The high throughput electrorotation chip and chamber may be used for cell-based screening for leading drug candidate molecules from a compound library, for high-throughput characterizing particle electric properties, and for high-throughput assaying molecular compositions of unknown solutions.

US Patent:

6716642, Apr 6, 2004

Filed:

Oct 10, 2000

Appl. No.:

09/685410

Inventors:

Lei Wu - San Diego CA
Xiaobo Wang - San Diego CA
Jing Cheng - Beijing, CN
Weiping Yang - San Diego CA
YuXiang Zhou - Beijing, CN
LiTian Liu - Beijing, CN
Junquan Xu - Beijing, CN

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

G01N 33543

US Classification:

436518, 42218615, 42218601, 422 50, 435 792, 435 15, 435 681, 435 71, 435 72, 435 6, 2503382, 436148

Abstract:

The present invention provides electromagnetic chips and electromagnetic biochips having arrays of individually addressable micro-electromagnetic units, as well as methods of utilizing these chips for directed manipulation of micro-particles and micro-structures such as biomolecules and chemical reagents.

US Patent:

6858439, Feb 22, 2005

Filed:

Oct 10, 2000

Appl. No.:

09/686737

Inventors:

Junquan Xu - Beijing, CN
Xiaobo Wang - San Diego CA, US
Jing Cheng - Beijing, CN
Weiping Yang - San Diego CA, US
Lei Wu - San Diego CA, US

Assignee:

Aviva Biosciences - San Diego CA

International Classification:

G01N033/543

US Classification:

436518, 436519, 436520, 436522, 436524, 436538, 436541, 436809, 436174, 436177, 436178, 436179, 436180, 435 72, 435 735, 435 733, 435 734, 435 736, 435 4, 435 5, 422 50, 204463, 204450, 204600, 204409, 204403

Abstract:

The present invention recognizes that separation of components of a sample facilitate, and are often necessary for, sample analysis. Dielectrophoretic separation provides an efficient, reliable, nondisruptive, and automatable method for the separation of moieties in a sample based on their dielectric properties. The present invention provides compositions and methods for enhancing the dielectrophoretic separation of one or more moieties in a sample. A first aspect of the present invention is a solution that when mixed with a sample, modifies at least one dielectric property of one or more components of the sample and has a conductivity such that one or more moieties of the sample can be separated using dielectrophoresis. Such solutions can be used in the analysis of samples on chips, and can be used in methods that use binding partners, including microparticles that can be translocated by dielectrophoretic forces, traveling-wave dielectrophoretic forces or magnetic forces.

US Patent:

6881314, Apr 19, 2005

Filed:

Oct 4, 2000

Appl. No.:

09/679023

Inventors:

Xiao-Bo Wang - San Diego CA, US
Jing Cheng - Beijing, CN
Lei Wu - San Diego CA, US
Junquan Xu - Fujian, CN

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

C02F001/40
C25B011/00
C25B013/00
C25B009/00
G01N027/27

US Classification:

204600, 204450, 204547

Abstract:

This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of matters utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

US Patent:

6900013, May 31, 2005

Filed:

Aug 25, 2000

Appl. No.:

09/648081

Inventors:

Guoqing Wang - Yi Wu, CN
Lei Wu - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Jing Cheng - Beijing, CN
WeiPing Yang - San Diego CA, US

Assignee:

AVIVA Biosciences Corporation - San Diego CA

International Classification:

C12Q001/68
C12P019/34
C12M001/34
C07H021/02
C07H021/04

US Classification:

435 6, 435 911, 435 912, 4352872, 536 231, 536 243, 536 2433

Abstract:

The present invention recognizes that identifying genes expressed during developmental processes, stress responses, and disease states can advance understanding of these biological functions, and can contribute to identifying targets for therapeutic drugs. In addition, the present invention recognizes that rapid and reliable profiling of genetic variations, such as mutations and SNPs, is of increasing importance to diagnostics, prognostics, forensics, heredity determinations, and pharmacogenetics. One aspect of the present invention provides a method of identifying one or more nucleic acid molecules that are expressed under a given set of conditions based on their complementarity to known sequences, or one or more mutations or SNPs in a population of nucleic acid molecules. The method includes: contacting at least one probe nucleic acid molecule with a survey population of nucleic acid molecules under conditions that promote nucleic acid hybridization to generate a probe-survey population mixture of nucleic acid molecules, treating the probe-survey population mixture of nucleic acid molecules with a nucleolytic activity, such that nucleolytic activity-sensitive nucleic acid molecules are digested, and contacting the resulting mixture of nucleolytic activity-protected nucleic acid molecules with a solid support comprising one or more attached nucleic acid molecules to generate attached nucleic acid molecule/nucleolytic activity-protected nucleic acid molecule complexes, and identifying one or more of the attached nucleic acid molecules or one or more of the nucleolytic activity-protected nucleic acid molecules in one or more attached nucleic acid molecule/nucleolytic activity-protected nucleic acid molecule complexes.