Kirk Max Ririe

US Patent:

6569627, May 27, 2003

Filed:

Mar 5, 2001

Appl. No.:

09/799160

Inventors:

Carl T. Wittwer - Salt Lake City UT
Kirk M. Ririe - Idaho Falls ID
Randy P. Rasmussen - Salt Lake City UT

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a double-stranded nucleic acid binding dye capable of producing a fluorescent signal related to the amount of the nucleic acid present in a sample, wherien the dye is selected from the group consisting of SYBRâ Green I and pico green.

US Patent:

7081226, Jul 25, 2006

Filed:

Jun 4, 1997

Appl. No.:

08/869275

Inventors:

Carl T. Wittwer - Salt Lake City UT, US
Kirk M. Ririe - Idaho Falls ID, US
Randy P. Rasmussen - Salt Lake City UT, US
David R. Hillyard - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

G01N 33/50
C12P 19/34

US Classification:

422 681, 422 50, 435 912

Abstract:

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.

US Patent:

7273749, Sep 25, 2007

Filed:

Aug 3, 2000

Appl. No.:

09/631339

Inventors:

Carl T. Wittwer - Salt Lake City UT, US
Kirk M. Ririe - Idaho Falls ID, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

C12M 1/34

US Classification:

4352872, 4352882, 4352887, 4353031, 435808, 422100, 422102

Abstract:

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.

US Patent:

7670832, Mar 2, 2010

Filed:

Aug 15, 2005

Appl. No.:

11/203947

Inventors:

Carl T. Wittwer - Salt Lake City UT, US
Kirk M. Ririe - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT
Idaho Technology, Inc. - Salt Lake City UT

International Classification:

C12M 1/00
C12Q 1/68

US Classification:

4352831, 435 6

Abstract:

The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.

US Patent:

7745205, Jun 29, 2010

Filed:

Aug 9, 2004

Appl. No.:

10/914648

Inventors:

Carl T. Wittwer - Salt Lake City UT, US
Kirk M. Ririe - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

C12M 1/34

US Classification:

4352872, 4352873, 4352887, 4353031, 4353033, 435808, 435809, 422 64

Abstract:

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.

US Patent:

8343754, Jan 1, 2013

Filed:

May 7, 2012

Appl. No.:

13/465364

Inventors:

Carl T. Wittwer - Salt Lake City UT, US
Kirk M. Ririe - Salt Lake City UT, US
Randy P. Rasmussen - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT
BioFire Diagnostics, Inc. - Salt Lake City UT

International Classification:

C12M 1/00

US Classification:

4352831

Abstract:

The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.

US Patent:

8394608, Mar 12, 2013

Filed:

May 8, 2006

Appl. No.:

11/913120

Inventors:

Kirk M. Ririe - Salt Lake City UT, US
Michael R. Newswander - Hyde Park UT, US
Randy P. Rasmussen - Salt Lake City UT, US
Mark Aaron Poritz - Salt Lake City UT, US
Stewart Benjamin Smith - Salt Lake City UT, US
David E. Jones - Clearfield UT, US
Gary Clark Kessler - Bountiful UT, US

Assignee:

BioFire Diagnostics, Inc. - Salt Lake City UT

International Classification:

C12P 19/34

US Classification:

435 912

Abstract:

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.

US Patent:

8409508, Apr 2, 2013

Filed:

Apr 23, 2003

Appl. No.:

10/512255

Inventors:

Kirk Max Ririe - Salt Lake City UT, US
Brent Farnes Higginson - Blackfoot ID, US
Abigail Bird - Salt Lake City UT, US

Assignee:

BioFire Diagnostics, Inc. - Salt Lake City UT

International Classification:

B01L 9/00

US Classification:

422 681

Abstract:

A device is provided for receiving a fluid sample. The device includes a fitment having a cavity formed therein. The cavity is provided under vacuum. The fitment also includes a port having a seal. The port is configured to provide fluid connection from an exterior surface of the fitment to the cavity upon opening of the seal. The device optionally includes a collapsible compartment coupled to the fitment and in fluid communication with the cavity.