John S Mudgett

US Patent:

20090191552, Jul 30, 2009

Filed:

Jun 22, 2007

Appl. No.:

12/227719

Inventors:

Kalpit A. Vora - Yardley PA, US
Julie A. DeMartino - Westfield NJ, US
John Stuart Mudgett - Basking Ridge NJ, US
Gene Porter - Neptune NJ, US
Gloria Wolfe - New York NY, US

International Classification:

C12Q 1/68
C12N 9/24
C07H 21/00
C12N 15/63
C12N 5/10

US Classification:

435 6, 435200, 536 232, 4353201, 435325

Abstract:

Described herein is a variant human acidic mammalian chitinase (AMCase) enzyme having improved stability and an isolated nucleic acid sequence which encodes the variant. The invention also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing recombinant variant AMCase polypeptide and its use in screening assays to identify modulators.

US Patent:

58500049, Dec 15, 1998

Filed:

Feb 28, 1997

Appl. No.:

8/808191

Inventors:

John MacMicking - New York NY
Carl Nathan - Larchmont NY
John S. Mudgett - Scotch Plains NJ

Assignee:

Cornell Research Foundation, Inc. - Ithaca NY
Merck & Co., Inc. - Rahway NJ

International Classification:

C12N 500
C12N 1500
A61K 4900

US Classification:

800 2

Abstract:

The present invention provides an inducible nitric oxide synthase ("iNOS")-deficient transgenic mouse, novel replacement vectors designed for the disruption of the iNOS gene, embryonic stem (ES) cells which are singly allelic relative to the deficient iNOS locus, a host cell line or cell clone carrying a congenitally altered iNOS gene, and a method of producing such a transgenic mouse. The iNOS-deficient transgenic mice can be used to evaluate and/or test their susceptibility to infectious or tumorigenic challenge, autoimmunity, septic shock and inflammatory and allergic diseases.