Jeffrey J Silva

US Patent:

7800055, Sep 21, 2010

Filed:

May 20, 2005

Appl. No.:

11/596584

Inventors:

Scott J. Geromanos - Middletown NJ, US
Jeffrey Cruz Silva - Beverly MA, US
Guo-Zhong Li - Westborough MA, US
Marc Victor Gorenstein - Needham MA, US

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

H01J 49/40

US Classification:

250288, 250281, 250282, 250283, 250286, 250287, 702 32, 702 23, 702 36, 210656, 2101982

Abstract:

LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions are grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group.

US Patent:

7851742, Dec 14, 2010

Filed:

May 20, 2005

Appl. No.:

11/596756

Inventors:

Scott J. Geromanos - Middletown NJ, US
Marc Victor Gorenstein - Needham MA, US
Jeffrey Cruz Silva - Beverly MA, US
Guo-Zhong Li - Westborough MA, US

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

H01J 49/00
B01D 59/44

US Classification:

250282, 250281, 250284, 250288

Abstract:

A system and method for mass analysis where a sample () is separated in a liquid chromatograph (), and subsequently analyzed in a mass spectrometer ().

US Patent:

D631537, Jan 25, 2011

Filed:

Sep 15, 2009

Appl. No.:

29/343553

Inventors:

Neil A. Sadler - Weehawken NJ, US
Jeffrey S. Silva - Jersey City NJ, US

Assignee:

McNeil-PPC, Inc. - Skillman NJ

International Classification:

2401

US Classification:

D24101

US Patent:

8030089, Oct 4, 2011

Filed:

Jul 16, 2004

Appl. No.:

10/893415

Inventors:

Scott Geromanos - Middletown NJ, US
Ashok R. Dongre - Newtown PA, US
Gregory Opiteck - Montreal, CA
Jeffrey Silva - Beverly MA, US

Assignee:

Micromass UK Limited

International Classification:

G01N 24/00

US Classification:

436173, 436 86

Abstract:

A method of identifying molecules of biological origin is disclosed. The molecules are identified and the basis of the accurately determined mass to charge ratio of the molecules and at least a further physico-chemical property such as elution time or charge state. Further physico-chemical properties may be used. The experimentally determined accurate mass and physico-chemical properties can then be compared with a look-up table of information. The look-up table may generated or physico-chemical properties of data in a conventional database may be calculated. The ability to recognize and preferably identify the same molecules in two different samples may be used to determine whether a particular biological molecules has been expressed differently in an experimental sample relative to a control sample.

US Patent:

8076635, Dec 13, 2011

Filed:

May 24, 2007

Appl. No.:

12/301469

Inventors:

Scott J. Geromanos - Middletown NJ, US
Guo-Zhong Li - Westborough MA, US
Jeffrey C. Silva - Beverly MA, US
Marc V. Gorenstein - Needham MA, US
Hans Vissers - Huizen, NL

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

H01J 49/00
H01J 49/40

US Classification:

250281, 250282, 702 22, 702 28

Abstract:

Embodiments of the present invention are directed to apparatus and methods for performing mass spectrometry. Data pair information is subjected to an ion audit process in which data pair information that relates to scored compounds is subtracted from the data pair information. The depleted information more readily reveals data pair information for compounds present with smaller signals.

US Patent:

8193485, Jun 5, 2012

Filed:

Oct 20, 2010

Appl. No.:

12/908235

Inventors:

Scott J. Geromanos - Middletown NJ, US
Marc Victor Gorenstein - Needham MA, US
Jeffrey Cruz Silva - Beverly MA, US
Guo-Zhong Li - Westborough MA, US

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

H01J 49/00
B01D 59/44

US Classification:

250282, 250281, 250284, 250288

Abstract:

Protein identification in a complex sample begins by selecting a database having proteins likely to be in the sample. In-silico digestion is performed and a target peptide is selected from produced peptides. The masses of the Y- and B-ion fragments of the target peptide are determined. These masses are used to search previously obtained low- and high-energy AMRTs obtained from LC/MS analysis of the complex sample for masses on the list. Any mass observed in the data within a detection threshold are considered a hit. If enough hits accumulate in a given retention time, the target peptide is identified as being in the sample. The list of peptides identified in the complex sample can be used to identify the proteins present in the sample, track the chromatographic retention times of peptides between samples, and quantitate the peptides and proteins present in complex samples.

US Patent:

8271207, Sep 18, 2012

Filed:

Jun 2, 2006

Appl. No.:

11/914578

Inventors:

Scott Geromanos - Middletown NJ, US
Jeffrey Cruz Silva - Beverly MA, US
Hans Vissers - Huizen, NL
Guo-Zhong Li - Westborough MA, US

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

G01N 30/04
G01N 30/00
G01N 30/26
G01N 30/72

US Classification:

702 23, 702 19, 702 27, 702 28

Abstract:

Absolute quantitation of protein in a sample is provided by comparing a sum or average of the N highest ionization intensities observed for peptides of a particular protein along with a calibration standard. The calibration standard can be in the form of a table generated by prior protein peptide analysis performed using one or more pre-determined proteins. The comparison is used to determine a corresponding absolute quantity of protein based on the observed sum or average of ionization intensities. A simple conversion factor can be applied to the calibration standard value to determine the absolute quantity of protein in the sample.

US Patent:

8373115, Feb 12, 2013

Filed:

Apr 30, 2012

Appl. No.:

13/460128

Inventors:

Scott J. Geromanos - Middletown NJ, US
Marc Victor Gorenstein - Needham MA, US
Jeffrey Cruz Silva - Beverly MA, US
Guo-Zhong Li - Westborough MA, US

Assignee:

Waters Technologies Corporation - Milford MA

International Classification:

B01D 59/44

US Classification:

250282, 250281, 250284, 250288

Abstract:

Protein identification in a complex sample begins by selecting a database having proteins likely to be in the sample. In-silico digestion is performed and a target peptide is selected from produced peptides. The masses of the Y- and B-ion fragments of the target peptide are determined. These masses are used to search previously obtained low- and high-energy AMRTs obtained from LC/MS analysis of the complex sample for masses on the list. Any mass observed in the data within a detection threshold are considered a hit. If enough hits accumulate in a given retention time, the target peptide is identified as being in the sample. The list of peptides identified in the complex sample can be used to identify the proteins present in the sample, track the chromatographic retention times of peptides between samples, and quantitate the peptides and proteins present in complex samples.