Brown University - Providence RI, US Toni-Marie Achilli - Providence RI, US Jacquelyn Youssef Schell - Providence RI, US
Assignee:
BROWN UNIVERSITY - Providence RI
International Classification:
C12Q 1/02
US Classification:
514 89, 435 29
Abstract:
The candidates are screened and then employed by administering to patients in need thereof of a drug candidate that affects heterotypic intercellular mechanotransduction. At least two types of cells are labeled with distinct intracellular fluorescent marker labels and combined to form a cell suspension and cultured to form a microtissue, such as spheroids. The cells or the spheroids are combined with a drug candidate, either before, during or after forming the spheroids. The distribution of the different cell types is compared to that of essentially the same suspension culture in the absence of the drug candidate. Alternatively, the cell power of cells cultured in a non-adherent mold that determines at least in part, the shape of microtissue formed is measured and compared with essentially the same cell suspension cultured in the same manner in the absence of a drug candidate. A patient in need thereof can be administered a drug identified as affecting heterotypic intercellular mechanotransduction. Alternatively, a macrotissue can be formed that is employed in therapeutic treatment of a patient in need thereof.
Jacquelyn Schell 1965 graduate of Binghamton North High School (thru 1982) in Binghamton, NY is on Classmates.com. See pictures, plan your class reunion and get caught up with ...