Ian R Greenshields

US Patent:

61668532, Dec 26, 2000

Filed:

Jan 9, 1997

Appl. No.:

8/781785

Inventors:

Mark A. Sapia - Canton CT
James C. Schaff - Cheshire CT
Ian R. Greenshields - Mansfield Center CT
Leslie M. Loew - W. Hartford CT
Frank R. Morgan - W. Hartford CT

Assignee:

The University of Connecticut - Storrs CT

International Classification:

G02B 2746
G06K 976
G06K 940

US Classification:

359559

Abstract:

An adaptive structure of a Wiener filter is used to deconvolve three-dimensional wide-field microscope images for the purposes of improving spatial resolution and removing out-of-focus light. The filter is a three-dimensional kernel representing a finite-impulse-response (FIR) structure requiring on the order of one thousand (1000) taps or more to achieve an acceptable mean-square-error. Converging to a solution is done in the spatial-domain and therefore does not experience many of the problems of frequency-domain solutions. Alternatively, a three-dimensional kernel representing an infinite-impulse-response (IIR) structure may be employed. An IIR structure typically requires fewer taps to achieve the same or better performance, resulting in higher resolution images with less noise and faster computations.