The present invention relates to compositions and methods for controlling nematode infestation of plants. In particular, the present invention provides vectors comprising sequences designed to control nematodes by RNA interference (RNAi) and transgenic plants transformed with such vectors.
Compositions And Methods For Controlling Parasitic Nematodes
Harold N. Trick - Olsburg KS, US Jiarui Li - Manhattan KS, US Timothy C. Todd - Manhattan KS, US
Assignee:
KANSAS STATE UNIVERSITY RESEARCH FOUNDATION - Manhattan KS
International Classification:
C12N 15/82 A01H 5/00
US Classification:
800301, 4353201
Abstract:
The present invention relates to compositions and methods for controlling nematode infestation of plants or animals. In particular, the present invention provides vectors comprising sequences designed to control nematodes by RNA interference (RNAi) and transgenic plants transformed with such vectors.
Mayetiola Destructor Susceptibility Gene One (Mds-1) And Its Application In Pest Management
Harold Trick - Olsburg KS, US Xiuming Liu - Manhattan KS, US
Assignee:
UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE - WASHINGTON DC KANSAS STATE UNIVERSITY RESEARCH FOUNDATION - Manhattan KS
International Classification:
C12N 15/82
US Classification:
800265, 800302, 800301, 800279
Abstract:
Plants having increased resistance to a pest relative to corresponding control plants are provided. The resistant plants have inhibited expression, activity, or function of a susceptibility gene, and thereby have decreased expression, activity, or function of the susceptibility gene in the presence of the pest as compared to a control plant in which the wild-type susceptibility gene is normally expressed in the presence of the pest. Methods of producing plants with increased resistance are also provided. The methods involve altering the expression, activity, or function of a susceptibility gene, such as Mds-1, in the plant. Isolated Mds-1 sequences are provided that can be manipulated to enhance pest resistance in modified plants. Modified plants, plant cells, tissues, seeds, and progeny are also provided having enhanced pest resistance.
Method For Transforming Plant Tissue By Sonication
John J. Finer - Wooster OH Harold N. Trick - Wooster OH
Assignee:
The Ohio State Research Foundation - Columbus OH
International Classification:
C12N 1300 C12N 1563
US Classification:
4351735
Abstract:
The present invention provides an efficient, cost effective method for transforming, including stably transforming, plants including monocots, dicots and gymnosperms. The method of the present invention, which is less labor intensive than typical conventional transformation methods, comprises combining the plant and a vector which contains the foreign nucleic acid to be introduced into the plant sample. Preferably the plant sample is sonicated in the presence of the vector. The vector is preferably non-tumor inducing bacteria, more preferably Agrobacterium. The sonication duration is less than 15 minutes, preferably less than 6 minutes more preferably 60 seconds or less. After sonicating the plant sample is cultured under conditions that induce morphogenesis to produce plant tissue that will mature into a transformed plant.
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