Ezra J Schildkraut

US Patent:

20090142811, Jun 4, 2009

Filed:

Sep 15, 2006

Appl. No.:

12/067000

Inventors:

Ira Schildkraut - Cerrillos NM, US
Ezra Schildkraut - Cerrillos NM, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12P 19/34
C12N 9/00
C07H 21/04
C12N 15/74

US Classification:

435 9152, 435183, 536 232, 4353201

Abstract:

Compositions that describe a thermostable DNA ligase isolated from sp. (strain 9 N-7) and methods for making und using the same are described. The thermostable DMA ligase depends on ATP and not NAD+ as a cofactor during ligation, and retains activity at 100 C.

US Patent:

20220333098, Oct 20, 2022

Filed:

Jun 22, 2022

Appl. No.:

17/846620

Inventors:

- Ipswich MA, US
Isaac B. Meek - Hopkinton MA, US
Dianne S. Schwarz - Watertown MA, US
Ezra Schildkraut - Boxford MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12N 15/10
C12P 19/34
C12N 9/22
C12N 9/96
C12N 15/115

Abstract:

Compositions and methods are provided for forming a single RNA polynucleotide from a plurality of DNA oligonucleotides in a single reaction chamber using combined reagents in a single step reaction. DNA polymerase, RNA polymerase and single stranded (ss) DNA oligonucleotides are combined where each DNA oligonucleotide has one or more sequence modules, wherein one sequence module in the first ss DNA oligonucleotide is complementary to a sequence module at the 3′ end of the second ss DNA oligonucleotide; and wherein a second module on the first ss DNA oligonucleotide is an RNA polymerase promoter sequence; and forming a single RNA polynucleotide, excluding the RNA promoter sequence, derived from the first and second DNA oligonucleotides