Dimitrios Angelo Kouzoukas

Dimitrios Kouzoukas Niles, IL

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Work:

Lexington Veterans Affairs Medical Center / University of Kentucky

Aug 2012 to 2000
Postdoctoral Scholar University of Kentucky, College of Medicine - Physiology
Lexington, KY
Jun 2011 to Aug 2011
Internship University of Iowa, Graduate College - Neurobiology
Iowa City, IA
Dec 2005 to Dec 2010
Research Assistant University of Iowa, Graduate College - Neuroscience
Iowa City, IA
2003 to 2005
Research Assistant University of Iowa, Graduate College - Neuroscience
Iowa City, IA
Jan 2001 to Dec 2002
Teaching Assistant University of Iowa, Graduate College - Neuroscience
Iowa City, IA
2001 to 2002
Research Assistant Loyola University Chicago, Stritch School of Medicine
Maywood, IL
Jan 1999 to May 1999
Teaching Assistant AMCOL International Corp. (Nanocor, Inc.), Research and Development
Hoffman Estates, IL
1996 to 1997
Laboratory Technician

Education:

University of Iowa, Graduate College
Iowa City, IA
2010
Ph.D. in Neurobiology Loyola University Chicago, College of Arts & Sciences
Chicago, IL
1997
B.S. in Biology & Psychology

Skills:

RESEARCH ANALYSIS & COMMUNICATION: Scientific writing and presentation; Over 15 years of solid experience with statistical analysis, data management, and experimental design; Proficient with common analysis (SPSS Statistics, GraphPad Prism, Excel, and ImageJ), citation (EndNote), graphics (Adobe Photoshop and Illustrator), and presentation software (PowerPoint); Neuroimaging and analysis; Familiar with Unix/Linux environments. MOLECULAR & CELLULAR PHYSIOLOGY: Generation and experimental manipulation of primary cell and organotypic culture systems; Live-cell microscopy with fluorescent probes and analysis including quantitation with ratiometric dyes; Examination of cell death and viability with multiple methods based on cell metabolism, plasma membrane integrity, and DNA binding; Extraction and analysis of proteins (ELISA, Western Blot, and multiplexed microsphere-based immunoassays) and nucleic acids (standard and qRT-PCR, including primer design) from cell cultures and tissues; Immunohistochemistry (frozen sections); BSL-2 and HPT methodology; Assay development, optimization, and troubleshooting; Rodent non-survival, survival, and multiple survival surgeries; Behavioral testing (von Frey, sucrose preference); Perfusion, fixation, tissue harvesting, and storage; Managing mouse colonies. RESEARCH AREAS: Neurodevelopment; Cell Biology; Calcium Signaling; Proteomics; Alcohol; Pain; Emotion Processing.