This invention provides an embryo specific promoter, e. g. derived from the 5′ regulatory region of an emb5 gene. Compositions comprising this promoter include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the embryo specific promoter sequences. The methods of the invention include the direct creation of transgenic plants with the emb5 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
High Lysine Maize Compositions And Event Ly038 Maize Plants
Mark A. Dizigan - Cranston RI, US Rebecca A. Kelly - South Kingstown RI, US Dale A. Voyles - Pawcatuck CT, US Michael Hans Luethy - Mystic CT, US Thomas M. Malvar - North Stonington CT, US Kathleen P. Malloy - Mystic CT, US
Disclosed herein are novel DNA constructs encoding dihydrodipicolinic acid synthase that are useful for generating transgenic events. Also provided are transgenic plants harboring such DNA constructs, the expression of which results in increased lysine in the plant or plant product. Also provided are maize plants having LY038 Event and progeny thereof having this event. Additionally disclosed are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site.
Methods And Compositions For Expression Of Transgenes In Plants
Alan L. Kriz - Gales Ferry CT, US Michael H. Luethy - Old Mystic CT, US Dale A. Voyles - Griswold CT, US
Assignee:
Dekalb Genetics Corporation - Dekalb IL
International Classification:
C12N 15/82 A01H 5/00
US Classification:
536 241, 800298, 4353201
Abstract:
The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.
High Lysine Maize Compositions And Methods For Detection Thereof
Mark A. Dizigan - Cranston RI, US Rebecca A. Kelly - South Kingstown RI, US Dale A. Voyles - Pawcatuck CT, US Michael Hans Luethy - Webster Groves MO, US Thomas M. Malvar - North Stonington CT, US Kathleen P. Malloy - Mystic CT, US
Assignee:
Monsanto Technology LLC - St. Louis MO
International Classification:
C07H 21/04 C12Q 1/68 C12P 19/34
US Classification:
536 231, 536 243, 536 2433, 435 6, 435 912
Abstract:
Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
Methods And Compositions For Expression Of Transgenes In Plants
Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus for transformation. Included in these transgene sequences are promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
Methods And Compositions For Expression Of Transgenes In Plants
Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus for transformation. Included in these transgene sequences are promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
Methods And Compositions For Expression Of Transgenes In Plants
Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus for transformation. Included in these transgene sequences are promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
High Lysine Maize Compositions And Methods For Detection Thereof
Mark A. Dizigan - Cranston RI, US Rebecca A. Kelly - South Kingstown RI, US Dale A. Voyles - Pawcatuck CT, US Michael Hans Luethy - Webster Groves MO, US Thomas M. Malvar - North Stonington CT, US Kathleen P. Malloy - Mystic CT, US
Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.
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