Collin M Fischer

Collin Fischer

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Collin Fischer Bridgeport, NY

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Work:

Department of Chemistry Syracuse University

2005 to 2000
Research Assistant Proteins and Nucleic Acid Purification

Mar 2010 to May 2010
Laboratory Instructor General Chemistry
Syracuse, NY
2007 to 2009
Laboratory Instructor Biomolecular Modeling

2007 to 2007
Laboratory Instructor Queens College

2001 to 2001
Undergraduate Research Assistant

Education:

Syracuse University, School of Arts and Sciences
Syracuse, NY
2005 to 2013
Ph.D. in Structural Biology, Biochemistry, and Biophysics Oswego State University
Oswego, NY
2002 to 2003
B.A. in Biological Sciences

Skills:

Recombinant protein expression and protein purification Protein solubility solutions Fast Protein Liquid Chromatography (FPLC) size exclusion chromatography (SEC), gel filtration (GF) and affinity chromatography (IMAC) AKTA and Unicorn (FPLC) Protein characterization Surface plasmon resonance (SPR) nucleic acid and proteins Biolayer interferometry (BLI) Label and label-free techniques Electrophoretic mobility shift assay (EMSA) Polymerase chain reaction (PCR) Site directed mutagenesis (SDM) Primer design Nucleic acid synthesis Molecular cloning including Gateway cloning Biomolecular modeling and molecular dynamics simulation MALDI-TOF mass spectrometry of purified proteins Native (PAGE) and denaturing polyacrylamide gel electrophoresis (SDS-PAGE) Western blot protein analysis Protein quantification Nanodrop, BCA, Bradford Isotopic labeling of protein for NMR Isothermal calorimetry Large scale cell culture Plasmid engineering HIV protein expression Tat, Ncp7 Bacteriorhodopsin expression Tm melting assays Proficient in many software packages (Excel, Word, PowerPoint, Blackboard, Clustal, Insight, Serial Cloner, etc.)

Collin Jon Fischer

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Specialties:

Internal Medicine
Cardiovascular Disease

Education:

Loma Linda University (2003)