Bernd Buehler

US Patent:

20090075259, Mar 19, 2009

Filed:

Aug 31, 2007

Appl. No.:

11/897886

Inventors:

Carsten-Peter Carstens - San Diego CA, US
Bernd Buehler - San Diego CA, US

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention provides methods, kits, and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of an analyte in a sample. In the methods of the invention a complex is formed between an analyte specific binding agent and an analyte. The analyte specific agents are coupled to an enzyme possessing an activity that produces a PCR template indicative of the presence of the analyte. Amplification and detection of the PCR template yields a sensitive and quantitative measurement of analyte concentration.