Arthur J Zaug

US Patent:

6696250, Feb 24, 2004

Filed:

Oct 10, 2000

Appl. No.:

09/686341

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Durham NC

Assignee:

Competitive Technologies, Inc. - Westport CT

International Classification:

C12Q 168

US Classification:

435 6, 435 9131, 536 232

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

US Patent:

49870718, Jan 22, 1991

Filed:

Dec 3, 1986

Appl. No.:

6/937327

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Boulder CO

Assignee:

University Patents, Inc. - Westport CT

International Classification:

C12D 1934
C12N 1500
C12N 910
C12N 912
C07H 1512
B01J 3100

US Classification:

435 91

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

US Patent:

53548558, Oct 11, 1994

Filed:

Feb 28, 1992

Appl. No.:

7/843737

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Boulder CO

International Classification:

C12N 1511

US Classification:

536 241

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

US Patent:

50932462, Mar 3, 1992

Filed:

Aug 3, 1990

Appl. No.:

7/562672

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Boulder CO

Assignee:

University Patents, Inc. - Westport CT

International Classification:

C12P 1934
C12N 1510
C12N 912

US Classification:

435 91

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribonsomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

US Patent:

55916104, Jan 7, 1997

Filed:

Jul 21, 1994

Appl. No.:

8/278624

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Boulder CO

Assignee:

University Patents, Inc. - Westport CT

International Classification:

C12P 1934
C07H 2102

US Classification:

435 9131

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.

US Patent:

50377469, Aug 6, 1991

Filed:

Mar 16, 1989

Appl. No.:

7/324385

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Durham NC

Assignee:

University Patents, Inc. - Westport CT

International Classification:

C12P 1934
C12N 912
C12N 1511
C07H 1512

US Classification:

435 91

Abstract:

A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C. sub. 5) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C. sub. 5 to generate chain lengths of 10 to 11 nucleotides; longer products are also generated but at reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C. sub. 5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template.

US Patent:

51167425, May 26, 1992

Filed:

Mar 24, 1989

Appl. No.:

7/328503

Inventors:

Thomas R. Cech - Boulder CO
Felicia L. Murphy - Boulder CO
Arthur J. Zaug - Louisville CO
Cheryl Grosshans - Denver CO

Assignee:

University Patents, Inc. - Westport CT

International Classification:

C12P 1934
C12N 1500
C12N 922

US Classification:

435 91

Abstract:

New RNA endoribonuclease ribozymes are found with new conditions to prevent mismatch cleavage and able to cleave RNA after 6 different sets of ribonucleotide 4 base sequences.

US Patent:

60251670, Feb 15, 2000

Filed:

Jun 5, 1996

Appl. No.:

8/671824

Inventors:

Thomas R. Cech - Boulder CO
Arthur J. Zaug - Louisville CO
Michael D. Been - Boulder CO

Assignee:

Competitive Technologies, Inc. - Westport CT

International Classification:

C12P 1934
C07H 2102

US Classification:

435 9131

Abstract:

RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribosomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.