Andrew B Cubitt

US Patent:

6403374, Jun 11, 2002

Filed:

Dec 16, 1999

Appl. No.:

09/465142

Inventors:

Roger Y. Tsien - La Jolla CA
S. James Remington - Eugene OR
Andrew B. Cubitt - San Diego CA
Roger Heim - Del Mar CA
Mats F. Ormö - Huddinge, SE

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 500

US Classification:

435325, 435410, 4352523, 43525233, 43525411, 4353201, 536 231, 536 236, 536 234

Abstract:

Engineered fluorescent proteins, nucleic acids encoding them and methods of use are provided.

US Patent:

6495664, Dec 17, 2002

Filed:

Jul 24, 1998

Appl. No.:

09/129192

Inventors:

Andrew B. Cubitt - San Diego CA

Assignee:

Aurora Biosciences Corporation - San Diego CA

International Classification:

C07K 100

US Classification:

530350, 530300, 435 4

Abstract:

The present invention includes a fluorescent compound that can detect an activity, such as an enzymatic activity, and exhibits quenching. The fluorescent compound can include a fluorescent protein, such as an Aequorea-related green fluorescent protein. The fluorescent compound can include a substrate site for an enzymatic activity such as a kinase activity, a phosphatase activity, a protease activity, and a glycosylase activity. The fluorescent compound of the present invention can be used to detect such enzymatic activities in samples, such as biological samples, including cells. The present invention also includes nucleic acids that encode the fluorescent compounds of the present inventions, and cells that include such nucleic acids or fluorescent compounds.

US Patent:

6780975, Aug 24, 2004

Filed:

Feb 5, 2002

Appl. No.:

10/071976

Inventors:

Roger Y. Tsien - La Jolla CA
S. James Remington - Eugene OR
Andrew B. Cubitt - San Diego CA
Roger Heim - Del Mar CA
Mats F. Ormö - Huddinge, SE

Assignee:

The Regents of the University of California - Oakland CA
Vertex Pharmaceuticals (San Diego) LLC - San Diego CA
State of Oregon Acting by and through the State Board of Higher Education on behalf of the University of Oregon - Eugene OR

International Classification:

C07K 1700

US Classification:

530350, 536 231

Abstract:

Engineered fluorescent proteins, nucleic acids encoding them and methods of use are provided.

US Patent:

7560287, Jul 14, 2009

Filed:

Aug 23, 2004

Appl. No.:

10/924232

Inventors:

Roger Y. Tsien - La Jolla CA, US
S. James Remington - Eugene OR, US
Andrew B. Cubitt - San Diego CA, US
Roger Heim - Del Mar CA, US
Mats F. Ormö - Huddinge, SE

Assignee:

The Regents of the University of California - Oakland CA
State of Oregon acting by and through the State Board of Higher Education on behalf of the University of Oregon - Eugene OR

International Classification:

G01N 21/64
G01N 33/52
C12P 17/06
A61K 38/00

US Classification:

436176, 436546, 435125, 4352851, 4351721, 435968, 435 71, 435 772, 530402

Abstract:

Engineered fluorescent proteins, nucleic acids encoding them and methods of use are provided.

US Patent:

7824850, Nov 2, 2010

Filed:

Jun 22, 2007

Appl. No.:

11/821562

Inventors:

Jeffrey Stack - San Diego CA, US
Michael Whitney - San Diego CA, US
Andrew B. Cubitt - San Diego CA, US
Brian Pollok - San Diego CA, US

Assignee:

Aurora Biosciences Corporation - San Diego CA

International Classification:

C12Q 1/00
C07K 14/00

US Classification:

435 4, 530350

Abstract:

This invention is directed towards methods of destabilizing proteins in living cells, and their use for the development of novel assays. In one embodiment, the invention comprises the use of non-cleavable multimerized ubiquitin fusion proteins to destabilize a target protein, such as a reporter moiety. In one aspect of this method the constructs also comprises a linker that operatively couples the reporter moiety to the multimerized ubiquitin fusion protein. In this embodiment, enzymatic modification of the linker results in a modulation of the coupling of the reporter protein to the multimerized ubiquitin domains resulting in a change in the stability of the reporter moiety. The level of the reporter moiety in the cell can then be used as a measure of the enzymatic activity in the cell. In another embodiment the invention provides for a generalized way of coordinately regulating the cellular concentration of a plurality of target proteins. In one aspect of this method, the target proteins are operatively coupled to a ubiquitin fusion protein via a linker containing a protease cleavage site.

US Patent:

8603950, Dec 10, 2013

Filed:

Feb 20, 2008

Appl. No.:

12/070904

Inventors:

Peter M. Bowers - Encinitas CA, US
Andrew B. Cubitt - San Diego CA, US
Robert A. Horlick - San Diego CA, US

Assignee:

AnaptysBio, Inc. - San Diego CA

International Classification:

C40B 40/06

US Classification:

506 26

Abstract:

This invention relates to methods for the generation of polynucleotide seed libraries and the use of these libraries in generating novel mutants of recombinant proteins and, more particularly, for generating focused libraries of recombinant human antibodies and screening for their affinity binding with target antigens.

US Patent:

7262005, Aug 28, 2007

Filed:

Feb 4, 2000

Appl. No.:

09/498098

Inventors:

Jeffrey Stack - San Diego CA, US
Michael Whitney - San Diego CA, US
Andrew B. Cubitt - San Diego CA, US
Brian Pollok - San Diego CA, US

Assignee:

Aurora Biosciences Corporation - San Diego CA

International Classification:

C12Q 1/68

US Classification:

435 6, 435325, 536 234

Abstract:

This invention is directed towards methods of destabilizing proteins in living cells, and their use for the development of novel assays. In one embodiment, the invention comprises the use of non-cleavable multimerized ubiquitin fusion proteins to destabilize a target protein, such as a reporter moiety. In one aspect of this method the constructs also comprises a linker that operatively couples the reporter moiety to the multimerized ubiquitin fusion protein. In this embodiment, enzymatic modification of the linker results in a modulation of the coupling of the reporter protein to the multimerized ubiquitin domains resulting in a change in the stability of the reporter moiety. The level of the reporter moiety in the cell can then be used as a measure of the enzymatic activity in the cell. In another embodiment the invention provides for a generalized way of coordinately regulating the cellular concentration of a plurality of target proteins. In one aspect of this method, the target proteins are operatively coupled to a ubiquitin fusion protein via a linker containing a protease cleavage site.

US Patent:

20020061546, May 23, 2002

Filed:

Jun 19, 2001

Appl. No.:

09/884681

Inventors:

Roger Tsien - La Jolla CA, US
Andrew Cubitt - San Diego CA, US

Assignee:

The Regents of the University of California

International Classification:

C12Q001/48
C12N009/12

US Classification:

435/015000, 435/194000

Abstract:

This invention provides assays for protein kinase activity using fluorescent proteins engineered to include sequences that can be phosphorylated by protein kinases. The proteins exhibit different fluorescent properties in the non-phosphorylated and phosphorylated states.