Alene A Campbell

US Patent:

20090286225, Nov 19, 2009

Filed:

Mar 11, 2009

Appl. No.:

12/402337

Inventors:

John H. Wheeler - Boulder CO, US
Michael Fiechtner - Poway CA, US
Jonathan D. Smith - Boulder CO, US
Duane Bush - Fort Collins CO, US
Alene A. Campbell - Boulder CO, US
Breanna C. Smith - Lyons CO, US

Assignee:

MicroPhageTM Incorporated - Longmont CO

International Classification:

C12Q 1/70
C12M 1/00

US Classification:

435 5, 4352877, 4352872, 4353031

Abstract:

Bacteriophage are combined with a test sample in an incubator, and the bacteriophage-exposed test sample is conjugated and applied to a sample pad in contact with a lateral flow strip to determine the presence or absence of a target bacterium. The conjugation may be performed in the sample pad or prior to application of the bacteriophage-exposed test sample to the pad. The incubator comprises a bacteriophage container and an incubation container separated by a valve. The test sample may be inserted into the incubation chamber using a swab or a rod with a piercing tip and a sample collection eye. The valve comprises a breakable stem. An antibiotic may be added to the test sample to determine the antibiotic resistance or susceptibility of the bacterium.