Akiko Koide

US Patent:

8263350, Sep 11, 2012

Filed:

Jun 29, 2010

Appl. No.:

12/826322

Inventors:

Shohei Koide - Chicago IL, US
Jin Huang - Beijing, CN
Akiko Koide - Chicago IL, US

Assignee:

The University of Chicago - Chicago IL

International Classification:

G01N 33/53

US Classification:

435 71, 435518

Abstract:

The invention provides a molecular affinity clamp. The architecture of the affinity clamp is modular with two biorecognition modules, each capable of binding a target motif. The first biorecognition module has a recognition domain that possesses inherent or natural specificity for the target motif. The second biorecognition module also has a recognition domain that binds the motif. The two biorecognition modules are tethered together either directly, e. g. , via a peptide bond between the two modules, or indirectly, e. g. , via a linker moiety or linker. The invention further provides a novel affinity ligand which is specifically bound by the molecular affinity clamps of the invention.

US Patent:

20070117966, May 24, 2007

Filed:

Apr 4, 2002

Appl. No.:

10/473770

Inventors:

Shohei Koide - Chicago IL, US
Stephen Dewhurst - Rochester NY, US
Akiko Koide - Chicago IL, US
Julie Richards - Rochester NY, US
Michelle Miller - Rochester NY, US

International Classification:

C07K 16/28

US Classification:

530388220

Abstract:

The present invention relates to a polypeptide monobody which includes a modified acid sequence and renders the polypeptide monobody able to bind selectively to αvβ3 integrin. Fusion proteins and conjugates which include the polypeptide monobody, as well as compositions containing the polypeptide monobody, fusion proteins, or conjugates are also disclosed. Uses thereof include: treating or preventing an αvβ3 integrin-mediated disease or disorder, inhibiting αvβ3 integrin activity, treating a cancerous or precancerous condition, imaging tissues using positron emission tomography or magnetic resonance imaging, assessing the metastatic characteristics of a tumor, and delivering DNA to a cell.

US Patent:

20110143963, Jun 16, 2011

Filed:

Nov 10, 2008

Appl. No.:

12/742014

Inventors:

Shohei Koide - Chicago IL, US
Akiko Koide - Chicago IL, US
Jin Huang - Chicago IL, US

Assignee:

University of Chicago - Chicago IL

International Classification:

C40B 40/10
C07K 19/00
B82Y 15/00

US Classification:

506 18, 530350, 977774

Abstract:

The invention provides a molecular affinity clamp. The architecture of the affinity clamp is modular with two biorecognition modules, each capable of binding a target motif. The first biorecognition module has a recognition domain that possesses inherent or natural specificity for the target motif. The second biorecognition module also has a recognition domain that binds the motif. The two biorecognition modules are tethered together either directly, e.g., via a peptide bond between the two modules, or indirectly, e.g., via a linker moiety or linker.

US Patent:

20140057807, Feb 27, 2014

Filed:

Aug 1, 2011

Appl. No.:

13/813409

Inventors:

Andreas Loew - Somerville MA, US
Brian Edward Vash - Combridge MA, US
Shohei Koide - Chicago IL, US
John Bernard Wojcik - Chicago IL, US
Akiko Koide - Chicago IL, US
Ryan Nicholas Gilbreth - Chicago IL, US

Assignee:

The University of Chicago - Chicago IL
Novartis AG - Basel

International Classification:

C07K 14/78

US Classification:

506 18, 530395, 506 26

Abstract:

The here described invention discloses a combination of a top and bottom loop binder library using the CD and the FG loops of a number of FnIII domains (FnIII) (e.g., FnIII, FnIIIand FnIII) together with the surface exposed residues of the beta-sheet. The invention also pertains to a method of forming a library of FnIII domain polypeptides useful in screening for the presence of one or more polypeptides having a selected binding or enzymatic activity.