Adam L Lowe

US Patent:

6451525, Sep 17, 2002

Filed:

Dec 3, 1998

Appl. No.:

09/204858

Inventors:

Andrew J. Blasband - Redwood City CA
Sandy M. Koepf - San Francisco CA
Adam L. Lowe - San Francisco CA

Assignee:

PE Corporation (NY) - Foster City CA

International Classification:

C12O 168

US Classification:

435 6, 435 911, 435 912, 536 2433, 536 2432, 536 231

Abstract:

A method for obtaining sequence information from a plurality of target polynucleotides in a sample is disclosed. In one embodiment, the method involves contacting a plurality of different sequence primers with a polynucleotide sample under conditions effective for the primers to anneal to primer-complementary regions in one or more target polynucleotides, to form one or more target-primer hybrid(s). Each different-sequence primer contains (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment. The hybrid(s) are contacted with a labeled nucleotide terminator in the presence of a primer-extending reagent, under conditions effective to append the base to an end of the annealed primer in the hybrid only when the base is complementary to a base in the target polynucleotide that is immediately adjacent to the end of the annealed primer, to form an extended hybrid mixture. The primers are then immobilized on an addressable array of immobilized, different tag complements, wherein each different tag complement contains a sequence that is complementary to the primer tag segments, under conditions effective to hybridize the primers to corresponding tag complements. The identification of a nucleotide base appended to at least one extended primer immobilized on the array allows determination of the presence of at least one target sequence in the sample.

US Patent:

6773887, Aug 10, 2004

Filed:

Oct 22, 2001

Appl. No.:

10/038520

Inventors:

Kenneth J. Livak - San Jose CA
Adam L. Lowe - San Francisco CA
Andrew J. Blasband - San Carlos CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912, 536 2433

Abstract:

Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle. In a second aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer-extension reagent; separating the target-primer hybrid from unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent and with a primer termination reagent, the primer termination reagent including a nucleotide terminator having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent and unreacted primer termination reagent; measuring a signal produced by the label; and repeating the above steps until a signal is detected indicating incorporation of the nucleotide terminator. The invention further includes kits useful for practicing the above methods.

US Patent:

7294464, Nov 13, 2007

Filed:

Jul 14, 2004

Appl. No.:

10/892403

Inventors:

Kenneth J. Livak - San Jose CA, US
Adam L. Lowe - San Francisco CA, US
Andrew J. Blasband - Redwood CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 221, 536 2433

Abstract:

Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle.

US Patent:

7414115, Aug 19, 2008

Filed:

Mar 30, 2007

Appl. No.:

11/731192

Inventors:

Kenneth J. Livak - San Jose CA, US
Adam L. Lowe - San Francisco CA, US
Andrew J. Blasband - Redwood CA, US

Assignee:

Applied Biosystems Inc. - Foster City CA

International Classification:

C07H 19/00
C07H 21/02
C07H 21/04
C12Q 1/68
C12P 19/34

US Classification:

536 221, 536 231, 536 2433, 435 6, 435 912

Abstract:

Disclosed is a kit useful for determining the number of repeat units in a repeat region of a target nucleic acid comprising: a plurality of different-sequence primers, each containing (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment, a first primer extension reagent; and a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a photodestructible label attached thereto.

US Patent:

20020143578, Oct 3, 2002

Filed:

May 24, 2001

Appl. No.:

09/864700

Inventors:

Louis Cole - Palo Alto CA, US
Adam Lowe - San Francisco CA, US
Lawrence Prensky - San Mateo CA, US
Jennifer Graham - Redwood City CA, US
George Morris - Campbell CA, US
Ronald Horn - Poway CA, US
John Vig - Carlsbad CA, US
Miriam Komaromy - Hillsborough CA, US
Kari Danziger - Foster City CA, US
John Shon - Palo Alto CA, US

International Classification:

G06F017/60

US Classification:

705/002000

Abstract:

A system for recording and assessing a person's inherited risk for disease. The system comprises a server for communicating with a communication device used by the person. The server comprises a risk assessment program that performs a risk assessment process. The process comprises the steps of: querying the person for family history information, receiving family history information inputted by the person, generating a family tree diagram based on the received family history information, transmitting the diagram to the communication device, querying the person for family medical history information concerning members of the person's family, receiving the family medical history information, generating a family medical tree diagram based on the received family medical history information, transmitting the diagram to the communication device, receiving a request to generate a risk assessment report, generating the risk assessment report, and transmitting it to the communication device.

US Patent:

59452846, Aug 31, 1999

Filed:

May 27, 1997

Appl. No.:

8/863437

Inventors:

Kenneth J. Livak - San Jose CA
Adam L. Lowe - San Francisco CA
Andrew J. Blasband - Redwood City CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C12Q 168
C07H 2102
C07H 2104

US Classification:

435 6

Abstract:

In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle. In a second aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer-extension reagent; separating the target-primer hybrid from unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent and with a primer termination reagent, the primer termination reagent including a nucleotide terminator having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent and unreacted primer termination reagent; measuring a signal produced by the label; and repeating the above steps until a signal is detected indicating incorporation of the nucleotide terminator. The invention further includes kits useful for practicing the above methods.

US Patent:

61687014, Jan 2, 2001

Filed:

Apr 30, 1999

Appl. No.:

9/303789

Inventors:

Shiaw-Min Chen - San Jose CA
Cheryl R. Heiner - La Honda CA
Adam L. Lowe - San Francisco CA

Assignee:

The Perkins-Elmer Corporation - Foster City CA

International Classification:

G01N 2726
G01N 27447

US Classification:

204456

Abstract:

The invention relates to improved methods and compositions for loading samples into an analytical instrument having a plurality of sample receiving loading ports. In a principle embodiment of the invention, first and second sample markers are added to sample to be loaded onto an analytical instrument having a plurality of sample receiving loading ports. The first and second samples are compounds are selected so as to produce a distinctive signal upon combination thus a sample containing a first sample marker and a sample containing a second marker are mistakenly loaded into the same sample receiving loading port, a detectable signal indicative of the misloading is produced.

US Patent:

63098296, Oct 30, 2001

Filed:

Dec 3, 1998

Appl. No.:

9/205114

Inventors:

Kenneth J. Livak - San Jose CA
Adam L. Lowe - San Francisco CA
Andrew J. Blasband - Redwood City CA

Assignee:

PE Corporation (NY) - Foster City CA

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. In a first aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer extension reagent; separating the target-primer hybrid and unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent, wherein at least one of the first or second primer extension reagents includes an extendible nucleotide having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent; measuring a signal produced by the label; treating the label so as to render the label undetectable; and repeating the above steps until the signal is substantially less than a signal detected in a previous cycle. In a second aspect, the method of the invention includes the steps of annealing a primer to a target nucleic acid; performing a first primer extension reaction using a first primer-extension reagent; separating the target-primer hybrid from unreacted first primer extension reagent; performing a second primer extension reaction using a second primer extension reagent and with a primer termination reagent, the primer termination reagent including a nucleotide terminator having a label attached thereto; separating the target-primer hybrid from unreacted second primer extension reagent and unreacted primer termination reagent; measuring a signal produced by the label; and repeating the above steps until a signal is detected indicating incorporation of the nucleotide terminator. The invention further includes kits useful for practicing the above methods.